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Cell Biology
Inhibitors
Phosphatase Inhibitor Cocktail (100X)
Phosphatase Inhibitor Cocktail (100X) from Cell Signaling Technology
Be the first to write a review!
Citations:
(96)
Supplier Page
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Cell Signaling Technology for
Phosphatase Inhibitor Cocktail (100X)
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Citations
Reviews
Product Specs
Item
Phosphatase Inhibitor Cocktail (100X)
Company
Cell Signaling Technology
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
5870S
Quantity
1 ml
Research Area
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Cell Signaling Technology
3 Trask Lane
Danvers
,
Massachusetts
01923
United States
Phone
: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488
Company Profile
Website:
http://www.cellsignal.com/
Website:
Technical Support
Citations (96)
(61)
GDF3 Protects Mice against Sepsis-Induced Cardiac Dysfunction and Mortality by Suppression of Macrophage Pro-Inflammatory Phenotype
Cells
January 3, 2020
Wang L, Li Y, Wang X, Wang P, Essandoh K, Cui S, Huang W, Mu X, Liu Z, Wang Y,
et al
.
Invitrogen
) containing 0.1 mM
PMSF, protease inhibitor
cocktail (Roche) and phosphatase
inhibitor
cocktail (
CST
,
5870s
). Protein concentrations were then measured by BCA
protein assay
method. Protein samples (20–50µg) were
(62)
Periostin interaction with discoidin domain receptor-1 (DDR1) promotes cartilage degeneration
PloS one
January 1, 2020
Han T, Mignatti P, Abramson SB, Attur M.
enzyme-linked immunosorbent assay
(ELISA) kits from R&D systems (Minneapolis, MN); Phosphatase Inhibitor Cocktail [#
5870
;
Cell signaling
, Danvers, MA, USA] and protease
inhibitor
cocktail (#539131, Calbiochem, San Diego,
(63)
EGFR targeting enhances the efficiency of chemotherapy through inhibiting IRE1α-XBP1s pathway in colorectal cancer cells
Journal of Cancer
January 1, 2020
Huo M, Zhao Y, Liu X, Gao Y, Zhang D, Chang M, Liu M, Xu N, Zhu H.
and then exposed in a dark room. RIPA (
9806S
), protease
inhibitor
(
5871S
), and phosphatase
inhibitor
(
5870S
) were purchased from
Cell Signaling Technology
.
Micro BCA™ Protein Assay Kit
for protein quantitation
(64)
Activation of endothelial ras-related C3 botulinum toxin substrate 1 (Rac1) improves post-stroke recovery and angiogenesis via activating Pak1 in mice
Experimental neurology
December 1, 2019
Bu F, Min JW, Munshi Y, Lai YJ, Qi L, Urayama A, McCullough LD, Li J.
phenylmethylsulfonyl fluoride (PMSF), protease
inhibitor
(04693116001, Roche) and phosphatase
inhibitor
(
5870
,
CST
) and sonicated for 10 seconds. Total protein was harvested from supernatant after cell lysate was
(65)
Gastrodin Ameliorates Motor Learning Deficits Through Preserving Cerebellar Long-Term Depression Pathways in Diabetic Rats
Frontiers in Neuroscience
November 22, 2019
Cheng-Kun Deng, Zhi-Hao Mu, Yi-He Miao, Yi-Dan Liu, Lei Zhou, Yong-Jie Huang, Fan Zhang, Yao-Yi Wang, Zhi-Hong Yang, Zhong-Yi Qian,
et al.
inhibitor
cocktail (
1:100
;
5871
;
Cell Signaling Technology
) and 1% phosphatase
inhibitor
cocktails (
1:100
;
5870
;
Cell Signaling Technology
) at 4°C. Homogenates were centrifuged at 12,000 × g for 10 min, and the supernatant
(66)
Multi-way and poly-phase wideband differential phase shifter based on metamaterial technology
AEU - International Journal of Electronics and Communications
October 1, 2019
A. Hamidia, M. Amin Honarvar, Bal S. Virdee
Results and Discussion As mentioned earlier the proposed DPS was designed and fabricated on Rogers Duroid
5870
substrate with a relative permittivity (εr) of 2.33, loss-tangent of 0.0012 and a thickness (h) of 1.575
(67)
NEAT1 is overexpressed in Parkinson's disease substantia nigra and confers drug‐inducible neuroprotection from oxidative stress
The FASEB Journal
October 1, 2019
Alon Simchovitz, Mor Hanan, Naomi Niederhoffer, Nimrod Madrer, Nadav Yayon, Estelle R. Bennett, David S. Greenberg, Sebastian Kadener, Hermona Soreq
lysis (20 min on ice) in standard RIPA buffer containing protease and phosphatase
inhibitors
(
5871
and
5870
, both diluted
1:100
;
Cell Signaling Technology
, Danvers, MA, USA), 150 µl per 6-well plate. Cells were
(68)
Early intervention with gastrodin reduces striatal neurotoxicity in adult rats with experimentally‑induced diabetes mellitus
Molecular Medicine Reports
February 14, 2019
Yu‑Han Qi et al.
cocktail (
1:100
; cat. no.
5871
;
CST
, Inc.) and 10X phosphatase
inhibitor
cocktail (
1:100
; cat. no.
5870
;
CST
, Inc.). Following complete lysis of the tissue, the homogenates were centrifuged at 3,028 x g for
(69)
Environmental Control of Astrocyte Pathogenic Activities in CNS Inflammation
Cell
January 24, 2019
Wheeler MA, Jaronen M, Covacu R, Zandee SEJ, Scalisi G, Rothhammer V, Tjon EC, Chao CC, Kenison JE, Blain M,
et al
.
cocktail (#
5871
,
Cell Signaling
,
1:100
working concentration) and phosphatase
inhibitor
cocktail (#
5870
,
Cell Signaling
,
1:100
working concentration).
Immunoprecipitation
for c-myc was performed for 4 hours
(70)
CORNER BENT INTEGRATED DESIGN OF 4G LTE AND MMWAVE 5G ANTENNAS FOR MOBILE TERMINALS
Progress In Electromagnetics Research M
January 1, 2019
Muhammad Idrees Magray, Gulur Sadananda Karthikeya, Khalid Muzaffar, Shiban Kishen Koul
(MS). Proposed 4G LTE and mmWave 5G MIMO antenna architecture is designed on a 10-mil thick Rogers
5870
substrate with dielectric constant (εr ) of 2.33 ± 0.02 and loss tangent of 0.0012. Substrate of low
(71)
Expression of cardiac inwardly rectifying potassium channels in pentylenetetrazole kindling model of epilepsy in rats
Cellular and Molecular Biology
December 31, 2018
Enes Akyüz, Pınar Mega Tiber, Merve Beker, Fahri Akbaş
Cell Signaling
;
9806
), containing protease and phosphatase
inhibitor
cocktail (
Cell Signaling
;
5871
,
5870
), proportional to sample
weights
. Tissue samples were homogenized and left for
incubation
to better
(72)
Hippo component YAP promotes focal adhesion and tumour aggressiveness via transcriptionally activating THBS1/FAK signalling in breast cancer
Journal of Experimental & Clinical Cancer Research
December 1, 2018
Jie Shen, Beibei Cao, Yatao Wang, Chenshen Ma, Zhuo Zeng, Liang Liu, Xiaolan Li, Deding Tao, Jianping Gong, Daxing Xie
phenylmethylsulfonyl fluoride and protein phosphatase
inhibitor
cocktail (
Cell Signaling Technology
, Cat. #
5870
) and was separated on 10% SDS-PAGE gels. After
electrophoresis
, the separated protein bands were transferred
(73)
Oxidative Stress in Cells with Extra Centrosomes Drives Non-Cell-Autonomous Invasion
Developmental cell
November 19, 2018
Arnandis T, Monteiro P, Adams SD, Bridgeman VL, Rajeeve V, Gadaleta E, Marzec J, Chelala C, Malanchi I, Cutillas PR,
et al
.
Mini Protease Inhibitor Cocktail,Roche,#11836153001 Phosphatase inhibitor Cocktail,
Cell Signaling
,#
5870
Bradford
Protein Assay
,
Bio-Rad
,#
5000006
Insulin-Transferrin-Selenium-Ethanolamine (ITS),Thermo Fisher
(74)
Molecular mechanisms of IL-33-mediated stromal interactions in cancer metastasis
JCI insight
October 18, 2018
Andersson P, Yang Y, Hosaka K, Zhang Y, Fischer C, Braun H, Liu S, Yu G, Liu S, Beyaert R,
et al
.
supplemented with a proteinase
inhibitor
(8340, MilliporeSigma) and a phosphatase
inhibitor
cocktail (
5870
,
Cell Signaling
). Protein concentrations were measured, and an equal amount of protein from each sample
(75)
The Human Cytomegalovirus Endoplasmic Reticulum-Resident Glycoprotein UL148 Activates the Unfolded Protein Response
Journal of virology
October 15, 2018
Siddiquey MNA, Zhang H, Nguyen CC, Domma AJ, Kamil JP.
phosphoeIF2␣(Ser51)], lysis buffer was further supplemented with 1⫻ phosphatase
inhibitor
cocktail (catalog number
5870S
;
Cell Signaling Technology
). Cell lysates were clarified by
centrifugation
at 18,000 ⫻ g for 30 min at
(76)
Ablation of endothelial VEGFR1 improves metabolic dysfunction by inducing adipose tissue browning
The Journal of experimental medicine
February 5, 2018
Seki T, Hosaka K, Fischer C, Lim S, Andersson P, Abe M, Iwamoto H, Gao Y, Wang X, Fong GH,
et al
.
the presence of a proteinase
inhibitor
(8340; Sigma-Aldrich) and a phosphatase
inhibitor
cocktail (
5870
;
Cell Signaling
). For
VEGFR2
phosphorylation analysis, an equal amount of total
protein extract
from
(77)
Evaluation of Hydrogels Presenting Extracellular Matrix-Derived Adhesion Peptides and Encapsulating Cardiac Progenitor Cells for Cardiac Repair
ACS Biomaterials Science & Engineering
January 8, 2018
Srishti Bhutani, Aline L.Y. Nachlas, Milton E. Brown, Tionne Pete, Christopher T. Johnson, Andrés J. García, Michael E. Davis
protease
inhibitor
(Sigma-Aldrich P2714) and phosphatase
inhibitor
cocktails (
Cell Signaling Technology
5870S
). The lysate was centrifuged at 10 000g for 10 min. The supernatant was stored at –80 °C until further
(78)
Temporal Control of Mammalian Cortical Neurogenesis by m
6
A Methylation
Cell
November 2, 2017
Yoon KJ, Ringeling FR, Vissers C, Jacob F, Pokrass M, Jimenez-Cyrus D, Su Y, Kim NS, Zhu Y, Zheng L,
et al
.
Technology,C9127-2s EdU,
Thermo Fisher Scientific
,
A10044
Phosphatase Inhibitor Cocktail,
Cell Signaling
,
5870
Protease Inhibitor Cocktail,Sigma,P8340 4x
Laemmli Sample Buffer
,
Bio-Rad
,1610747
Methylene blue
,
Sigma-Aldrich
,M9140 A83-01,
Stemcell Technologies
(79)
Characterization of Growth Hormone Resistance in Experimental and Ulcerative Colitis
International Journal of Molecular Sciences
September 23, 2017
Christoffer Soendergaard, Peter Helding Kvist, Peter Thygesen, Mats Reslow, Ole Haagen Nielsen, John Joseph Kopchick, Thomas Lindebo Holm
added protease and phosphatase
inhibitors
(Thermo Scientific, 186209) (
Cell signalling
technology,
5870
). The lysate was sonicated and cleared by
centrifugation
and loaded on homemade 8%
SDS-PAGE gels
following
(80)
Zika-Virus-Encoded NS2A Disrupts Mammalian Cortical Neurogenesis by Degrading Adherens Junction Proteins
Cell stem cell
September 7, 2017
Yoon KJ, Song G, Qian X, Pan J, Xu D, Rho HS, Kim NS, Habela C, Zheng L, Jacob F,
et al
.
Technology,C9127-2s EdU,
Thermo Fisher Scientific
,
A10044
Phosphatase Inhibitor Cocktail,
Cell Signaling
,
5870
Protease Inhibitor Cocktail,Sigma,P8340 4×
Laemmli Sample Buffer
,
Bio-Rad
,1610747 A83-01,
Stemcell Technologies
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Phosphatase Inhibitor Cocktail (100X) from Cell Signaling Technology
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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